Neubauer improved counting chamber with CE (1 pc)

Large square: 1 mm²
Group square: 0.04 mm²
Smallest square: 0.025 mm²

This is the preferred counting chamber today. The chamber depth is 0.100 mm. The grid division of this chamber has 3 by 3 large squares, each with an area of 1 mm². The 4 corner squares are used for leukocyte counting. The large square in the middle is further subdivided into 5 by 5 group squares with a side length of 0.2 mm each and an area of 0.04 mm² each. The group squares are in turn subdivided into 16 smallest squares of 0.0025 mm² each. Five of these group squares are used for erythrocyte counting.

Special attention should be paid to the fact that the chamber has triple boundary lines on all sides, of which the middle line is to be regarded as the actual measuring line. This is important for assessing whether cells lying in the boundary area should be counted or not.