Amikacin AMI 30 µg (10x 50 sheets, BD)

BACKGROUND
Antibiotic disc diffusion susceptibility test discs are used for semi-quantitative susceptibility testing of common, fast-growing and certain fastidious bacteria against antibiotics using the agar diffusion method.

TEST PRINCIPLE
Filter paper discs impregnated with defined amounts of antibiotics are applied to the surface of a suitable medium (e.g., Mueller-Hinton agar). The culture medium must first be inoculated with a standardized inoculum (EUCAST recommendation: McFarland 0.5) of a fresh pure culture of the bacterial strain to be tested. After a 24-hour incubation, growth is checked, the inhibition zones around the test discs are measured, and compared with the clinical breakpoints for the various antibiotics tested to determine susceptibility (S - Susceptible at standard exposure, I - potentially susceptible at increased exposure, R - Resistant). The diameter of the inhibition zones corresponds to the susceptibility of the bacterial strain tested to the antibiotic concentration at the edge of the inhibition zone.

You can find various service documents, such as templates with inhibition zone boundaries for easy evaluation of the agar diffusion test, a collection of guidelines, and application videos in our media library.

Content and Storage:
Cartridges with 50 test discs each.
Store at 2-8°C.

Note: Cartridges of the BD system are only compatible with BD dispensers (Becton Dickinson).

As an alternative to agar diffusion, we recommend agar dilution, which can be partially automated with the MICRONAUT system, for example, and for which there are increasingly more and more accurate clinical breakpoints. Since EUCAST 10.0 (2020) at the latest, certain antibiotics can only be tested by agar dilution, and no longer by agar diffusion (!). We would be happy to advise you on this and help you find the best solution for you.