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Mueller-Hinton II Agar Prepared - 20 pcs.
MD2472
€8,95
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€8,95
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Müller-Hinton Agar (MH-Agar) is a non-synthetic, economical, non-selective medium for determining the susceptibility of rapidly growing aerobic organisms to antimicrobial agents.
According to RiliBÄK / EUCAST, up to 6 antibiotics can be tested simultaneously on these 90mm plates.
The MH medium can also be used for conducting the inhibition test.
As a synthetic alternative, Iso-Res medium ("Sensitivity Agar") is available.
For the agar diffusion test, according to the McKirby method, 2-3 colonies of a bacterium from a pure culture are dissolved in 2 ml of 0.85% NaCl. If a densitometer is available, standardization to McFarland 0.5 can be performed (but is not strictly required if deviating). From this inoculum, 100 µl are pipetted onto an MH plate and spread evenly with a fresh inoculation loop. Subsequently, antibiotic discs (individually or with a dispenser) are placed. When loading the discs, the applied standard (EUCAST or CLSI) must be considered. For reading according to EUCAST, we provide reading templates as a service material.
According to RiliBÄK / EUCAST, up to 6 antibiotics can be tested simultaneously on these 90mm plates.
The MH medium can also be used for conducting the inhibition test.
As a synthetic alternative, Iso-Res medium ("Sensitivity Agar") is available.
For the agar diffusion test, according to the McKirby method, 2-3 colonies of a bacterium from a pure culture are dissolved in 2 ml of 0.85% NaCl. If a densitometer is available, standardization to McFarland 0.5 can be performed (but is not strictly required if deviating). From this inoculum, 100 µl are pipetted onto an MH plate and spread evenly with a fresh inoculation loop. Subsequently, antibiotic discs (individually or with a dispenser) are placed. When loading the discs, the applied standard (EUCAST or CLSI) must be considered. For reading according to EUCAST, we provide reading templates as a service material.
For the inhibition test, Bacillus subtilis is used as the germ. You can also purchase the corresponding strain from us. It can be cost-effectively maintained using the cryobank method and cultivated as needed.
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