Colistin COL 10 µg (5x 50 sheets, Mast)

Background:
Antibiotic test discs are used to determine the semi-quantitative susceptibility testing of common, fast-growing, and certain fastidious bacteria to antibiotics using the agar diffusion method.

Test Principle:
Filter paper discs impregnated with defined amounts of antibiotics are applied to the surface of an appropriate medium (e.g., Mueller-Hinton agar). The culture medium must first be inoculated with a standardized inoculum (EUCAST recommendation: McFarland 0.5) of a fresh pure culture of the bacterial strain to be tested. After 24 hours of incubation, growth is checked, the zones of inhibition around the test discs are measured, and compared with the clinical breakpoints for the various antibiotics tested to determine susceptibility (S - Susceptible to standard exposure, I - possibly susceptible to increased exposure, R - Resistant). The diameter of the zones of inhibition corresponds to the susceptibility of the tested bacterial strain to the antibiotic concentration at the edge of the zone of inhibition.

You can find various service documents, such as templates with inhibition zone boundaries for easy evaluation of the agar diffusion test, a collection of guidelines, and application videos in our  Media Library.

Content and Storage:

• Cartridges with 50 test discs each.
• Store at 2-8° C.

Note: MAST system cartridges are only compatible with MAST dispensers.

Alternatively to agar diffusion, we recommend agar dilution, which can be semi-automated, for example, with the  MICRONAUT system , and for which there are increasingly more and more accurate clinical breakpoints, i.e., at least since EUCAST 10.0 (2020), certain antibiotics can only be tested by agar dilution, and no longer by agar diffusion (!). We would be happy to advise you on this and help you find the best solution for you.
 

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