Ampicillin-Sulbactam AMS 10/10µg (1x 50 discs, Mast)

Background:
Antibiotic test discs are used to determine the semi-quantitative susceptibility testing of common, fast-growing, and certain fastidious bacteria to antibiotics using the agar diffusion method.

Test Principle:
The filter paper discs impregnated with defined amounts of antibiotics are applied to the surface of an appropriate medium (e.g., Mueller-Hinton agar). The culture medium must first be inoculated with a standardized inoculum (EUCAST recommendation: McFarland 0.5) of a fresh pure culture of the bacterial strain to be tested. After 24 hours of incubation, growth is checked, the zones of inhibition around the test discs are measured, and compared with the clinical breakpoints for the various antibiotics tested to determine susceptibility (S - Susceptible at standard exposure, I - Susceptible with increased exposure, R - Resistant). The diameter of the zones of inhibition corresponds to the susceptibility of the tested bacterial strain to the antibiotic concentration at the edge of the zone of inhibition.

You can find various service documents, such as templates with inhibition zone limits for easy evaluation of the agar diffusion test, a collection of guidelines, and application videos in our media library.

Contents and Storage:

• Cartridges each containing 50 test discs.
• Store at 2-8° C.

Note: MAST system cartridges are only compatible with MAST dispensers.

As an alternative to agar diffusion, we recommend agar dilution, which can be partially automated, for example, with the MICRONAUT system, and for which there are increasingly more and more precise clinical breakpoints. This means that at least since EUCAST 10.0 (2020), certain antibiotics can only be tested using agar dilution and no longer using agar diffusion (!). We would be happy to advise you and help you find the best solution for you.