Amoxicillin AMO 25 µg (5x 50 sheets, Mast)

Background:
Antibiotic test discs are used for semi-quantitative susceptibility testing of common, fast-growing, and certain fastidious bacteria to antibiotics using the agar diffusion method.

Test Principle:
Filter paper discs impregnated with defined amounts of antibiotics are placed on the surface of an appropriate medium (e.g., Mueller-Hinton agar). The culture medium must first be inoculated with a standardized inoculum (EUCAST recommendation: McFarland 0.5) of a fresh pure culture of the bacterial strain to be tested. After 24 hours of incubation, growth is checked, inhibition zones around the test discs are measured, and compared with clinical breakpoints for the different antibiotics tested to determine susceptibility (S - Susceptible at standard exposure, I - Susceptible with increased exposure, R - Resistant). The diameter of the inhibition zones corresponds to the susceptibility of the tested bacterial strain to the antibiotic concentration at the edge of the inhibition zone.

You can find various service documents, such as templates with inhibition zone boundaries for easy evaluation of the agar diffusion test, a collection of guidelines, and application videos in our media library.

Content and Storage:

• Cartridges with 50 test discs each.
• Store at 2-8°C.

Note: MAST system cartridges are only compatible with MAST dispensers.

As an alternative to agar diffusion, we recommend agar dilution, which can be partially automated with systems such as the MICRONAUT system . There are increasingly more and more precise clinical breakpoints for this method, meaning that since EUCAST 10.0 (2020), certain antibiotics can only be tested using agar dilution and no longer by agar diffusion (!). We would be happy to advise you on this and help you find the best solution for your needs.
 

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