{"product_id":"who-puffer-z-spermienfixierung-100ml","title":"WHO Buffer for Sperm Fixation (100ml)","description":"\u003cdiv class=\"productDescription\"\u003e\n\u003cp\u003eApplication of the laboratory reagent:\u003c\/p\u003e\n\u003cp\u003eThe WHO sperm fixative solution simplifies the procedure for determining sperm concentration.\u003c\/p\u003e\n\u003cp\u003eManual examination is performed in a 100 µm deep \u003ca href=\"https:\/\/www.springermedizin.de\/emedpedia\/detail\/lexikon-der-medizinischen-laboratoriumsdiagnostik\/zaehlkammer?epediaDoi=10.1007%2F978-3-662-49054-9_3345\"\u003ecounting chamber\u003c\/a\u003e, preferably the improved Neubauer counting chamber.\u003c\/p\u003e\n\u003cp\u003eStandard procedure: Dilution with a bicarbonate-formalin solution, which serves to fix the cells. Gentian violet or trypan blue can be added to this solution to make the spermatozoa easier to visualize.\u003c\/p\u003e\n\u003cp\u003eModified procedure with this product: Dilution with fixation and staining for easier identification of spermatozoa takes place in one step.\u003cbr\u003e\u003cbr\u003eFor both procedures:\u003c\/p\u003e\n\u003cp\u003e-Only intact sperm with head and tail (consisting of midpiece, principal piece, and endpiece) should be counted;\u003cbr\u003e-The (pre-)dilution should be chosen so that at least 200 sperm are found in each of the two counting chamber sides at 400x magnification.\u003c\/p\u003e\n\u003cp\u003eFurther information on the WHO-compliant performance of the spermiogram can be found here, for example:\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.springermedizin.de\/emedpedia\/detail\/andrologie\/untersuchung-des-ejakulates?epediaDoi=10.1007%2F978-3-662-61904-9_9\"\u003ehttps:\/\/www.springermedizin.de\/emedpedia\/detail\/andrologie\/untersuchung-des-ejakulates?epediaDoi=10.1007%2F978-3-662-61904-9_9\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eDownloads:\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/aurosan.sharepoint.com\/:b:\/s\/AUROSAN_4Y\/IQDCCFyWFQ3KSbtKkvCg3kAPARyrxcbc8doLwnmDP5alzwI?e=VQDRXa\" title=\"Sicherheitsdatenblatt\"\u003eSafety Data Sheet\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003cbr\u003e","brand":"Diagonal GmbH \u0026 Co. KG","offers":[{"title":"Default Title","offer_id":56933253382476,"sku":"MD2499","price":41.45,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0828\/1056\/9036\/files\/BC_Upload_811211f0-7c3e-49b4-8805-fbc1cf4f40ae.jpg?v=1773255553","url":"https:\/\/aurosan-shop.de\/en\/products\/who-puffer-z-spermienfixierung-100ml","provider":"aurosan-shop.de","version":"1.0","type":"link"}